Be careful with your principal components

Be careful with the primers when screening your clones by polymerase chain reaction.

Polymerase chain reaction (PCR) is a powerful tool with many different applications in Molecular Biology and related disciplines. Amplification of the inserts cloned into a vector directly from the bacterial colony is one of them. This technique allows a fast screening of many different clones in order to detect which one of them contains the desired construction. The primers used to direct thi...

Be careful with Exp-function method

An application of the Exp-function method to search for exact solutions of nonlinear differential equations is analyzed. Typical mistakes of application of the Exp-function method are demonstrated. We show it is often required to simplify the exact solutions obtained. Possibilities of the Exp-function method and other approaches in mathematical physics are discussed. The application of the sing...

Correcting hypotension: be careful!

To cite: Foran E, Abkur TM. BMJ Case Rep Published online: [please include Day Month Year] doi:10.1136/ bcr-2015-212904 DESCRIPTION A 42-year-old woman presented with a 2-day history of vomiting. She was drowsy, dehydrated, tachycardic and hypotensive with a blood pressure (BP) of 52/37. The immediate management included infusion of 4 L of normal saline and inotropic support. Investigations sho...

Let's be careful out there.

If we are to change the way these services are regulated, we first must know what the trade-offs are. Consumers need to know what they are giving up if they exchange the certainty of their regulated, monopoly provider for the harsh world of the competitive marketplace. Make no mistake, there is a vast difference in cultures between a monopoly, regulated utility run with the ratepayer uppermost ...

Can We Be Too Careful?